19.  Deposition of Bone-Like Hydroxyapatite on Grafted Fibroin Silk Fibers

Authors: M.R. Tudora, C. Zaharia, A. Diacon, C. N. Degeratu, E. Mircea, C. Andronescu, C. Cincu, N. Preda and I. Enculescu


Abstract:

Silk is naturally occurring protein polymer produced by a wide variety of insects and spiders. Bombyx mori silk, a member of Bombycidae family has been used as biomedical suture material for centuries. These types of proteins usually exhibit important mechanical properties. Because of these impressive mechanical properties, this family of proteins provides an important set of material options in the fields of controlled release of biomaterials and scaffolds for tissue engineering. The grafting of silk with acidic groups may lead to the formation of hydroxyapatite by incubation in medium that mimic blood plasma. The idea that the acidic functions stimulate the formation of hydroxyapatite gained attention in the last 15 years in the literature. Natural fibrous polymers were chemically modified to mimic the behaviour of bone proteins responsible for mineralization. In this respect, 2-acrylamido-2-methylpropane sulphonic acid (AMPSA), 2- hydroxyethyl methacrylate -2-acrylamido-2-methylpropane sulphonic acid (HEMA-AMPSA) and diethylamino ethyl methacrylate (DEAEMA) were grafted onto silk fibroin by cerium ammonium nitrate initiation. The resulted polymers were characterized by FTIR-ATR and XPS spectroscopy to prove the grafting reactions. The biomineralization capacity of the grafted fibroin was evaluated by incubation in simulated body fluid solutions (SBF1x). SEM analysis showed the presence of hydroxyapatite deposits onto the surface of the grafted fibroin samples and the value of Ca/P ratio was very close to 1.67 from bone hydroxyapatite. The new synthesized biomaterials prove to have real mineralization ability and could be a potential bone substitute in the future.


Keywords: silk, graft polymerization, hydroxyapatite, biomineralization, simulated body fluid (SBF1x)

PDF Version

 
 
Issues

Articles in Press
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2016
Volume 61(75), Issue 2, 2016
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2016
Volume 61(75), Issue 1, 2016
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2015
Volume 60(74), Issue 2, 2015
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2015
Volume 60(74), Issue 1, 2015
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2014
Volume 59(73), Issue 2, 2014
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2014
Volume 59(73), Issue 1, 2014
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2013
Volume 58(72), Issue 2, 2013
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2013
Volume 58(72), Issue 1, 2013
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2012
Volume 57(71), Issue 2, 2012
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2012
Volume 57(71), Issue 1, 2012
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2011
Volume 56(70), Issue 2, 2011
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2011
Volume 56(70), Issue 1, 2011
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2010
Volume 55(69), Issue 2, 2010
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2010
Volume 55(69), Issue 1, 2010
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2009
Volume 54(68), Issue 2, 2009
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2009
Volume 54(68), Issue 1, 2009
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2008
Volume 53(67), Issue 1 - 2, 2008
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2007
Volume 52(66), Issue 1 - 2, 2007
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2006
Volume 51(65), Item 1 - 2, 2006
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)


2005
Volume 50(64), Item 1 - 2, 2005
Chem. Bull. `POLITEHNICA` Univ. (Timisoara)



   
 
 
  © 2024 Industrial Chemistry and Environmental Engineering Faculty