5.  Long-Term Exposure Stability of Sol-Gel Immobilized Lipases in Organic Solvents

Authors: P. Borza, F. Peter, I. Hulka and C. Paul


Abstract:

Stability of lipases from Pseudomonas fluorescens (Amano AK), Burkholderia cepacia (Amano PS) and Candida antarctica B (Cal B), immobilized by sol-gel entrapment and sol-gel entrapment combined with adsorption on Celite 545, was investigated by long-term incubation in seven different solvents. The immobilization has been carried out using a ternary silane precursors system consisted of phenyl-trimethoxysilane, vinyl-trimethoxysilane and tetramethoxysilane, at 1.6/0.4/1 molar ratio. The immobilized biocatalysts were incubated in various organic solvents for 7 days, at 25°C. The activity and enantioselectivity were assayed in the acylation reaction of 2-hexanol with vinyl acetate. The activity of the immobilized biocatalysts was not significantly altered by the tested organic solvents, while the enantioselectivity values of all immobilized enzymes were higher compared to the native ones. In the case of Cal B lipases the operational stability was excelent as well, the transesterification activity remaining unchanged during 14 reuse cycles.


Keywords: lipase, sol-gel entrapment, stability, organic solvent, reuse

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